The text field allows two input formats:
- Non-Fasta, if the sequence code (DNA or Protein) is pasted directly into the input field.
- Fasta format, if multiple sequences should be processed.
In the lower section of the page it is possible to refine your query by adjustment of BLAST parameters:
If the sequences are provided in FASTA format, the last checkbox needs to be checked. Otherwise an error will occur!
Set an eValue threshold.
- BLAST algorithm
Choose between blastn, blastx and tblastx.
Choose the initial Wordsize from 1 to 50.
Select if transcripts with multiple reads (contigs), singletons or both should be used as reference BLAST database.